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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Dec 5;1863(2):177–190. doi: 10.1016/j.bbalip.2017.12.002

Fig. 4.

Fig. 4

Cellular cholesterol negatively regulates NgBR expression

A, B: HepG2 cells in serum-free and complete (10% serum) medium received atorvastatin treatment at the indicated concentrations for 16 h, respectively; C–G: HepG2 cells in serum-free medium received the following treatment for 16 h: β-cyclodextrin (β-CD) (C, D), LDL or water soluble cholesterol (Chol/CD) (E), β-CD (2.5 mM) or β-CD plus Chol/CD (F), and β-CD or Chol/CD (G), at the indicated concentrations. Cellular TC levels were determined by TC assay kit (A, C). Expression of NgBR protein was determined by Western blot (B, D–F). Expression of GGPS1 mRNA was determined by real time RT-PCR (G). *: P < 0.05 vs. control or as indicated in the corresponding group; H, I: HepG2 cells were transfected with scrambled siRNA (Control) or HMGCR siRNA (HMGCR) followed by treatment with atorvastatin (10 μM) for 5 h. Cellular free cholesterol and expression of HMGCR and NgBR protein were determined by filipin staining and Western blot, respectively. All the experiments were repeated 3 times.