Table 1.
Sequences of primers for real time RT-PCR assay.
| Gene | Species | Sense | Anti-sense |
|---|---|---|---|
| ACC1 | Human | 5′-GCCATTGGGATTGGGGCTTAC-3′ | 5′-CCCGCCCGAGGACTTTGTTG-3′ |
| Mouse | 5′-GAAGTCAGAGCCACGGCACA-3′ | 5′-GGCAATCTCAGTTCAAGCCAG-3′ | |
| ATGL | Human | 5′-AGCATCTGCCAGTACCTGGTGAT-3′ | 5′-ACCTGCTCCGGCAGCCTGG-3′ |
| Mouse | 5′-GAGCCCCGGGGTGGAACAAGAT-3′ | 5′-AAAAGGTGGTGGGCAGGAGTA-3′ | |
| CGI-58 | Human | 5′-GATCAGCAAGGTCTGGTCGT-3′ | 5′-TAGGTGGATTCTTGGCTGCT-3′ |
| Mouse | 5′-TGTGCAGGACTCTTACTTGGCAGT-3′ | 5′-GTTTCTTTGGGCAGACCGGTTT-3′ | |
| CH25H | Human | 5′-ATGTTGACCACGTGGAAGGT-3′ | 5′-TGGGAACTGTTTTCTTTGGG-3′ |
| Mouse | 5′-TGGAGTCAATGACACTGGGA-3′ | 5′-TGTTGTTTTTGCAGCCTGAC-3′ | |
| CYP27A1 | Human | 5′-AGCTGCGCTTCTTCTTTCAG-3′ | 5′-GGCCCTAAGTAGGACATCCA-3′ |
| Mouse | 5′-GGGCACTAGCCAGATTCACA-3′ | 5′-CTATGTGCTGCACTTGCCC-3′ | |
| CYP46A1 | Human | 5′-CTGTCCCAGGCAGTGAAACT-3′ | 5′-AATGCTCTCCCGGACCTC-3′ |
| Mouse | 5′-AACACATCTTGGAGCACACG -3′ | 5′-GCTATGAGCACATCCCCG-3′ | |
| FASN | Human | 5′-AACTCCAAGGACACAGTCACCAT-3′ | 5′-CAGCTGCTCCACGAACTCAAA-3′ |
| Mouse | 5′-CTGCGATGAAGAGCATGGTTT-3′ | 5′-CCATAGGCGATTTCTGGGAC-3′ | |
| GGPS1 | Human | 5′-GCCCACAGCATCTATGGAAT-3′ | 5′-GCGGGTAAAAAGCTTCACTG-3′ |
| GAPDH | Human | 5′-GGTGGTCTCCTCTGACTTCAACA-3′ | 5′-GTTGCTGTAGCCAAATTCGTTGT-3′ |
| Mouse | 5′-ACCCAGAAGACTGTGGATGG-3′ | 5′-ACACATTGGGGGTAGGAACA-3′ | |
| HSL | Human | 5′-GAAGCCTTTGAGATGCCACT-3′ | 5′-AGATGAGCCTGACGAGGAC-3′ |
| Mouse | 5′-GCCGGTGACGCTGAAAGTGGT-3′ | 5′-CGCGCAGATGGGAGCAAGAG-3′ | |
| NgBR | Human | 5′-AGCCTCGTGGTGTGGTGTA-3′ | 5′-GCCCAGAAGTTCTTGCTGTT-3′ |
| NPC2 | Human | 5′-TGGGCTCACATTCACTTCCT-3′ | 5′-AGCTACATTCCTGCTCCTGG-3′ |
| SCD1 | Human | 5′-TGGGTTGGCTGCTTGTG-3′ | 5′-GCGTGGGCAGGATGAAG-3′ |
| Mouse | 5′-TGGGTTGGCTGCTTGTG-3′ | 5′-GCGTGGGCAGGATGAAG-3′ | |
| SREBP1c | Human | 5′-GGATTGCACTTTCGAAGACATG-3′ | 5′-AGCATAGGGTGGGTCAAATAGG-3′ |
| Mouse | 5′-GCGCCATGGACGAGCTG-3′ | 5′-TTGGCACCTGGGCTGCT-3′ | |
| TGH | Human | 5′-GCTCCAGCATCTCTGTGGTT-3′ | 5′-GGAACAGACGACACTGTCAAA-3′ |
| Mouse | 5′-TCCTGGGGTCTATCGTCTGA-3′ | 5′-CCCATCTGTAATCGTGTCCC-3′ | |
| LDLR | Mouse | 5′-GAGGAACTGGCGGCTGAA-3′ | 5′-GTGCTGGATGGGGAGGTCT-3′ |
| MTTP | Mouse | 5′-CTGGATCTCCATATTGGCCT-3′ | 5′-TGATGTCCAAAATGCTGTCG-3′ |
| VLDLR | Mouse | 5′-TGCACACGAAGTCAGACTCA-3′ | 5′-CCTGCTGTGGAAATGTGATG-3′ |
ACC1: acetyl-CoA carboxylase 1; ATGL: adipose triglyceride lipase; CGI-58: comparative gene identification-58; CH25H: cholesterol 25-hydroxylase; CYP27A1: cytochrome P450 family 27 subfamily A member 1 (cholesterol 27-hydroxylase); CYP46A1: cytochrome P450 family 46 subfamily A member 1 (cholesterol 24-hydroxylase); FASN: fatty acid synthase; GGPS1: geranylgeranyl diphosphate synthase 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HSL: hormone-sensitive lipase; LDLR: low density lipoprotein receptor; MTTP: microsomal triglyceride transfer protein; NgBR: Nogo-B receptor; NPC2: Niemann-Pick type C2; SCD-1: stearoyl-CoA desaturase 1; SREBP1c: sterol-responsive element binding protein 1c; TGH: triacylglycerol hydrolase; VLDLR: very low density lipoprotein receptor.