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. Author manuscript; available in PMC: 2018 Jan 3.
Published in final edited form as: ACS Appl Mater Interfaces. 2016 Aug 2;8(32):20540–20548. doi: 10.1021/acsami.6b04679

Figure 7.

Figure 7

Average neurite length and ratio of neurite-bearing cells. Quantification of average axon length was performed by analyzing maximal intensity images (A) in all three planes (B) for both PSHU–PNIPAAm–RGD and PSHU–PNIPAAm cultures at 3, 5, and 7 day time points (C). The ratio of neurite-bearing cells (F) was quantified by calculating the number of cells bearing neurites in a visual frame (E) and dividing by the total number of cells within that frame (D). Numbers are plotted for both PSHU–PNIPAAm–RGD and PSHU–PNIPAAm over the 7 day culture period. Statistical significance between groups was determined by ANOVA. *p < 0.05; **p < 0.005. Three images were taken from each of at least three different samples from three separate RGC isolations. The scale bar is 200 μms. Day 5 cultures are shown to provide examples for quantification (A–E).