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. 2017 Nov 26;8(65):108292–108302. doi: 10.18632/oncotarget.22660

Figure 3. In vitro effects of the anti-SLPI compound C74 and its effects on the metastasis and primary tumors in vivo.

Figure 3

A. The molecular structure of the selected compound C74. B. C74 inhibition of SLPI expression by MDA-MB-468 cells. Cells were treated with C74 (0.31 to 5 µM) for 24 hours, and SLPI expression was quantified using luciferase activity driven by the SLPI promoter. C. Viability of HUVEC, MDA-MB-468 and 4T1 cancer cells treated with C74 for 24 hours evaluated using a MTT assay. D., E. Anti-SLPI compound C74 suppressed tumor growth and metastasis of 4T1 TNBC in mice. (D) 4T1 cells were injected in the mammary fat pad of female BALB/c mice and the mice were treated with C74 (25 mg/kg) daily for 12 days (from day 6 to day 17) via intraperitoneal injection. Error bars represent SEM; n = 8 mice/group, * P < 0.05. (E) Quantification of the numbers of large (>1 mm, black bar) and total lung metastases (white bar) observed after harvesting lungs on day 23. Error bars represent SEM; n = 8 mice/group, * P < 0.05.