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. 2017 Nov 27;8(65):108316–108332. doi: 10.18632/oncotarget.22718

Figure 2. In vitro analysis of PAX8 knockdown models.

Figure 2

PAX8 was stably knocked down using short hairpin RNAs (shRNAs). PAX8 gene expression in knockdown and control lines in a. HeyA8 and b. IGROV1 ovarian cancer cell lines. Knockdown of PAX8 protein was confirmed by immunofluorescent staining in c. HeyA8 and d. IGROV1 cells. Nuclei were counterstained with Hoescht DNA stain. 200X magnification is shown. e.-f. Anchorage independent growth assays in (e) HeyA8 and (f) IGROV1 PAX8 knockdown models. (g-h) Anchorage dependent growth assays in g. HeyA8 and h. IGROV1 models. Data shown are mean ± standard deviation, and are representative of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, two-tailed paired T-test. In panels (g) and (h) T-tests values (two tailed, paired) for knockdown lines compared to shScr lines are indicated.