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. 2017 Jul 7;8(65):108430–108450. doi: 10.18632/oncotarget.19086

Figure 6. GW6471 effects on lipid droplets and cholesterol content in normoxic and hypoxic GSCs.

Figure 6

A. LD stained with BODIPY 493/503 in treated GSCs shows a significant decrease of LDs in both conditions. Nuclei are stained with Dapi. Bar = 25µm. B. Western blotting analysis for FABP7 and PPARγ shows that only in hypoxic condition at 16 µM, the treatment was able to decrease FABP7 and to increase PPARγ. Data are means ± SD of 3 different experiments. ++, p < 0.001 hypoxia vs the respective control C. PPARγ immunofluorescence in treated GSCs supports the western blotting results. Nuclei are stained with Dapi. Bar = 25µm. D. qRT-PCR analysis of the enzymes of the mevalonate pathway in treated GSCs. Data are means ± SD of 3 different experiments. +++, p < 0.0001 hypoxia vs the respective control,***,p < 0.0001, normoxia with respect to the respective control. E. Cholesterol content in treated GSCs shows a significant decrease of cholesterol content upon treatment in both conditions. Data are means ± SD of 3 different experiments. +, p < 0.01, hypoxia vs the respective control; ***, p < 0.0001, normoxia with respect to the respective control.