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. 2017 Nov 20;8(65):109027–109042. doi: 10.18632/oncotarget.22561

Table 2. Antibodies for immunohistochemistry and immunofluorescence.

Antibodies (Clone, Isotype) Dilution Antigen retrieval Source
Rabbit anti-IgA Ready-to-use Trypsin Nichirei
Mouse anti-IgG (A57H, IgM) Ready-to-use Trypsin Nichirei
Rabbit anti-IgE × 100 Trypsin Dako
rabbit anti-IgM × 60 Trypsin Covance
Rabbit anti-human TSLP (ab47943, IgG) 10 μg/ml Immunosaver Abcam
Goat anti-human TSLPR (IgG) 10 μg/ml Immunosaver R&D
Rat anti-human AID (EK2 5G9, IgG2b) × 100 Immunosaver Cell Signaling
Goat anti-human TGF-β1 (IgG) 10 μg/ml Immunosaver R&D
Rat anti-human BAFF (ab16081, IgG2b) × 100 Immunosaver Abcam
Mouse anti-human APRIL (IgG1) × 100 Immunosaver Enzo
Mouse anti-CD10 (56C6, IgG1) Ready-to-use EDTA Nichirei
Mouse anti-CD21 (1F8, IgG1) × 40 Immunosaver Dako
Mouse anti-CD23 (1B12, IgG1) × 30 Immunosaver Novocastra
HRP-donkey F (ab´)2 anti-goat IgG × 100 Not use Abcam
Goat anti-rabbit secondary antibody × 100 Not use Jackson
Biotinylated anti-sheep IgG × 100 Not use Vector
Rhodamine-donkey anti-mouse IgG × 100 Not use Jackson
FITC-donkey anti-mouse IgG × 100 Not use Jackson
FITC-goat anti-rabbit IgG × 100 Not use Jackson
FITC-donkey anti-goat IgG × 100 Not use Beckman Coulter
Alexa Fluor 568-goat anti-rat IgG × 100 Not use Invitrogen
Fluor 555-goat anti-rabbit IgG × 100 Not use Invitrogen