Figure 8. PKM2 expression increases chromosomal aberrations following DDR.

Three representative metaphase spreads of MCF7 cells reconstitutively expressing empty vector (EV), PKM2-WT or PKM2-Del^515-520 mutant either untreated or 24hr following etoposide treatment. FISH-mediated whole chromosomal staining was used to identify aberrations of chromosome 5. Bottom panel shows the quantification of total chromosome aberrations/metaphase (30 metaphases scored/group). Data are mean ± SD of triplicates in an independent experiment, which were repeated at least for three times with the same results. ^**p< 0.01 versus PKM2-WT.