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. 2017 Nov 24;8(65):109161–109174. doi: 10.18632/oncotarget.22644

Figure 1. IL-22 induces the anti-microbial peptide and chemokines from HaCaT cells.

Figure 1

(A) HaCaT cells were treated with 20ng ml/1 of either IL-22 for 12 or days 24 hours, then the cells were harvested. The relative expressions of anti-microbial peptides and chemokines were measured via real-time RT–PCR. Data are expressed as the means ± SD (i.e. n=3) and compared with those of the ‘no treatment’ group on each of the experimental days. Data were analyzed by Two-Way Analysis of Variance (ANOVA). (B) Immunoassays of cell lysates from HaCaT cells stimulated with IL-22 revealed increases in NLRP3, the mature form of caspase-1 and the active form of IL-1β. HaCaT cells were treated with IL-22 (20 ng ml/1) for 24 h at 37°C. The cells were lysed and the whole-cell extracts were detected with anti-NLRP3, anti-caspase-1 and anti-IL-1β antibodies. Representative blots of three independent experiments are shown. Results were normalized against β-actin expression.