Skip to main content
. 2017 Nov 24;8(65):109161–109174. doi: 10.18632/oncotarget.22644

Figure 7. Conditional medium inhibits NHEM migration.

Figure 7

(A) NHEM cells were seeded in the upper chamber of transwell. CM-12 and CM-24 inhibited NHEM migration. Scale bar, 50 μm. (B) After 24 h, the migrated NHEMs were quantified. (C) Western blot assays were performed to examine Rab7, Rab27a and Rab17 expression levels, Total cellular proteins (20 μg/lane of NHEM) were subjected to 12 % SDS-PAGE. Results were normalized against GAPDH expression. Results shown are means ± SD (i.e. n=3). Data were analyzed by One-Way Analysis of Variance (ANOVA) followed by post hoc Tukey test. **P < 0.01, compared with control.