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. 2017 Nov 27;8(65):109301–109318. doi: 10.18632/oncotarget.22673

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Copyright: © 2017 Wei et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A and B) Fluorescence-activated cell sorting and flow cytometry. CRC cells HT-29 (A) and Caco-2 (B) were separated into CD133⁺CD44⁺ and CD133^-CD44^- CRC cell population. (C and D) The proliferation rates of CD133⁺CD44⁺ and CD133^-CD44^- HT-29 (C) or Caco-2 (D) cells from days 1 through 4 after seeding (^**: P<0.01). (E) The colony formation assay indicated that more colonies were formed in the CD133⁺CD44⁺ CRC cell population than in the CD133^-CD44^- CRC cell population (^**: P<0.01). (F and G) Tumorsphere formation of CD133⁺CD44⁺ or CD133^-CD44^- CRC cell population from HT-29 (F) or Caco-2 (G) cells. (H) The cell numbers per tumorsphere in CD133⁺CD44⁺ and CD133^-CD44^- HT-29 or Caco-2 cells (^**: P<0.01).