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. 2017 Nov 27;8(65):109301–109318. doi: 10.18632/oncotarget.22673

Table 1. Sequences of the oligonucleotides used for qPCR and chromatin immunoprecipitation (ChIP).

qPCR 5’→ 3’
Ascl2 F: 5’-CGTGAAGCTGGTGAACTTGG-3’
R: 5’-GGATGTACTCCACGGCTGAG-3’
CD133 F: 5’-TTCTTGACCGACTGAGACCCA-3’
R: 5’-TCATGTTCTCCAACGCCTCTT-3’
CD44 F: 5’-CTGCCGCTTTGCAGGTGTA-3’
R: 5’-CATTGTGGGCAAGGTGCTATT-3’
Bmi1 F: 5’-CGTGTATTGTTCGTTACCTGGA-3’
R: 5’-TTCAGTAGTGGTCTGGTCTTGT-3’
C-myc F: 5’-GGCTCCTGGCAAAAGGTCA-3’
R: 5’-CTGCGTAGTTGTGCTGATGT-3’
Oct4 F: 5’-CTGGAGAAGGAGAAGCTGGA-3’
R: 5’-CAAATTGCTCGAGTTCTTTCTG-3’
KLF5 F: 5’-AAGGAGTAACCCCGATTTGG-3’
R: 5’-TGGCTTTTCACCAGTGTGAG-3’
YAP1 F: 5’-TAGCCCTGCGTAGCCAGTTA-3’
R: 5’-TCATGCTTAGTCCACTGTCTGT-3’
MST1 F: 5’-AGTGCCAAAGGAGTGTCAATAC-3’
R: 5’-GGATTCCTGGCGTTTCAGTTTC-3’
β-actin F: 5’-TTGCCGACAGGATGCAGAA-3’
R: 5’-GCCGATCCACACGGAGTACT-3’
ChIP 5’→ 3’
ChIP1 F: 5’-TCAGACCAGGGACAGGGAG-3’
R: 5’-GGCCGGAAGAGTAGCACC-3’
ChIP2 F: 5’-CTGGCACAAGGCGGTCGG-3’
R: 5’-TGAGGCGGGGTGGGAGGTT-3’
ChIP3 F: 5’-CCCAGTAGATTAACGCACAG-3’
R: 5’-GCCCCATTGAGGAAGC-3’
ChIP4 F: 5’-GCCACAGTTTTCCCCGTCGCCTCC-3’
R: 5’-GCATCCACCCGCCCGCTCCA-3’
GAPDH (ChIP for
positive control)
F: 5’-TACTAGCGGTTTTACGGGCG-3’
R: 5’-TCGAACAGGAGGAGCAGAGAGCGA-3’