Table 1. Sequences of the oligonucleotides used for qPCR and chromatin immunoprecipitation (ChIP).
qPCR | 5’→ 3’ |
---|---|
Ascl2 | F: 5’-CGTGAAGCTGGTGAACTTGG-3’ R: 5’-GGATGTACTCCACGGCTGAG-3’ |
CD133 | F: 5’-TTCTTGACCGACTGAGACCCA-3’ R: 5’-TCATGTTCTCCAACGCCTCTT-3’ |
CD44 | F: 5’-CTGCCGCTTTGCAGGTGTA-3’ R: 5’-CATTGTGGGCAAGGTGCTATT-3’ |
Bmi1 | F: 5’-CGTGTATTGTTCGTTACCTGGA-3’ R: 5’-TTCAGTAGTGGTCTGGTCTTGT-3’ |
C-myc | F: 5’-GGCTCCTGGCAAAAGGTCA-3’ R: 5’-CTGCGTAGTTGTGCTGATGT-3’ |
Oct4 | F: 5’-CTGGAGAAGGAGAAGCTGGA-3’ R: 5’-CAAATTGCTCGAGTTCTTTCTG-3’ |
KLF5 | F: 5’-AAGGAGTAACCCCGATTTGG-3’ R: 5’-TGGCTTTTCACCAGTGTGAG-3’ |
YAP1 | F: 5’-TAGCCCTGCGTAGCCAGTTA-3’ R: 5’-TCATGCTTAGTCCACTGTCTGT-3’ |
MST1 | F: 5’-AGTGCCAAAGGAGTGTCAATAC-3’ R: 5’-GGATTCCTGGCGTTTCAGTTTC-3’ |
β-actin | F: 5’-TTGCCGACAGGATGCAGAA-3’ R: 5’-GCCGATCCACACGGAGTACT-3’ |
ChIP | 5’→ 3’ |
ChIP1 | F: 5’-TCAGACCAGGGACAGGGAG-3’ R: 5’-GGCCGGAAGAGTAGCACC-3’ |
ChIP2 | F: 5’-CTGGCACAAGGCGGTCGG-3’ R: 5’-TGAGGCGGGGTGGGAGGTT-3’ |
ChIP3 | F: 5’-CCCAGTAGATTAACGCACAG-3’ R: 5’-GCCCCATTGAGGAAGC-3’ |
ChIP4 | F: 5’-GCCACAGTTTTCCCCGTCGCCTCC-3’ R: 5’-GCATCCACCCGCCCGCTCCA-3’ |
GAPDH (ChIP for positive control) |
F: 5’-TACTAGCGGTTTTACGGGCG-3’ R: 5’-TCGAACAGGAGGAGCAGAGAGCGA-3’ |