Table 2. The primary antibodies used in the experiment.
| Primary antibody | Company | Dilution |
|---|---|---|
| Mouse monoclonal IgG to Ascl2 | Millipore(MAB4418) | WB: 1:500 |
| Rabbit polyclonal IgG to CD133 | Proteintech(18470-1-AP) | WB: 1:500 |
| Rabbit polyclonal IgG to CD44 | Proteintech(15675-1-AP) | WB: 1:200 |
| Rabbit polyclonal IgG to Bmi1 | Proteintech (10832-1-AP) | WB: 1:500 |
| Rabbit polyclonal IgG to C-myc | Proteintech(10828-1-AP) | WB: 1:500 |
| Rabbit polyclonal IgG to Oct4 | Proteintech(11263-1-AP) | WB: 1:500 |
| Rabbit polyclonal IgG to KLF5 | Proteintech(21017-1-AP) | WB: 1:500 |
| Rabbit polyclonal IgG to KLF5 | Santa Cruz Biotechnology(sc-22797) | IP: 1:50; ChIP |
| Rabbit polyclonal IgG to YAP1 | Proteintech(13584-1-AP) | WB: 1:500 |
| Rabbit monoclonal IgG to YAP1 | Cell Signaling(#14074) | IP: 1:50; ChIP |
| Rabbit polyclonal IgG to MST1 | Cell Signaling(#3682) | WB: 1:1000 |
| Rabbit monoclonal IgG to Phospho-YAP(Ser127) | Cell Signaling(#13008) | WB: 1:1000 |
| Mouse monoclonal IgG to β-actin | Proteintech(66009-1-Ig) | WB: 1:4000 |
| Rabbit polyclonal IgG to Lamin B1 | Proteintech(12987-1-AP) | WB: 1:5000 |