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. 2017 Nov 27;8(65):109301–109318. doi: 10.18632/oncotarget.22673

Table 3. The primer sequences used in the each Ascl2 promoter fragment.

pGL3-Ascl2-promotor 5’→ 3’ Length of product
-2588/+620 F: 5’-ggggtacc TCCGGAGATCTTACCA-3’
R: 5’-cggctagc CGCGCCTGCATCCAC-3’		 3208 bp
-628/+620 F:5’-ggggtacc GACCGTCCTGGCACAAG-3’
R: 5’-cggctagc CGCGCCTGCATCCAC-3’		 1248 bp
-82/+620 F: 5’-ggggtacc GATCTTGCGCGCCTC-3’
R: 5’-cggctagc CGCGCCTGCATCCAC-3’		 702 bp

The underlined parts in the primers are KpnI and NheI adaptors.