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. 2017 Nov 25;8(65):109358–109369. doi: 10.18632/oncotarget.22678

Figure 1. Cx43 (GJAI1) mRNA is elevated in JIMT-1 cells compared to SK-BR-3 cells but Cx43 protein is not.

Figure 1

(A) GJA1 expression is associated with reduced relapse free survival (RFS) in HER2+/ErbB2 patients. Gene probe GJA1 201667_at was used for analysis with HER2+ status set to “positive” and ER status set to “negative” yielding n=137 patient samples with available clinical data containing the selected events. A total of n=68 patients were scored as “low” GJA1 and n=69 were scored as “high” GJA1. Analysis tool automatically removed redundant samples and excluded any biased arrays. The probe expression range was classified as 73-16584 with a cutoff value of 2320 used for analysis. HR=1.96, logrank p-value=0.012. (B) Quantitative RealTime PCR assessment of Cx43 (GJA1) mRNA expression levels in SK-BR-3 compared with JIMT-1 breast cancer cells. JIMT-1 cells had ∼8-fold higher levels of GJA1 mRNA in relation with SK-BR-3. GJA1 levels were normalized to GAPDH. Student’s T-test indicated a p-value of p=0.01, n=3. (C) Western blot analysis of endogenous Cx43 protein levels in SK-BR-3 and JIMT-1 cells.