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. 2017 Nov 29;8(65):109692–109702. doi: 10.18632/oncotarget.22766

Figure 2. Atsttrin exhibited therapeutic effect in murine disc ex vivo.

Figure 2

(A) After culture the mouse IVD with TNF-α (50 ng/ml) in the presence or absence of Atsttrin (1 ug/ml) respectively and Safranin O staining was performed. (B) Chondrostaining density analysis shown Atsttrin attenuated the loss of cartilage tissue in IVD induced by TNF-α. (C) After culture the mouse IVD with TNF-α (50 ng/ml) in the presence or absence of Atsttrin (1 ug/ml) respectively and HE staining was performed. TNF-α accelerated the loss of the height and nucleus pulposus in IVD, while co-cultured with Atsttrin alleviated this structural degeneration. Abbreviation: NP: nucleus pulposus; AF: annulus fibrosus. Scale bar=200 um. The values are mean ± SEM of at least 3 independent experiments; ***P<0.001 VS control group, #P<0.05 VS TNF-α treatment group.