Figure 5. Significant correlation of MLKL expression with susceptibilities of ovarian cancer cells to ceramide nanoliposomes-induced cell death.
A, Extracted cellular proteins from the indicated ovarian cancer cell lines were subjected to immunoblot analysis with antibodies for MLKL, RIPK-1, RIPK-3 and β-actin. Equal amounts of protein were loaded in each lane. Three independent experiments were performed and representative images are shown. The intensity of MLKL protein expression was quantified using Image Lab. IC50 values for ceramide nanoliposomes are plotted with MLKL protein expression (B), RIPK1 protein expression (C) and RIPK3 protein expression (D). R2 values were determined by GraphPad prism.