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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2017 Nov 15;17(1):26–38. doi: 10.1158/1535-7163.MCT-17-0580

Figure 4. Epigenetic regulation of PEG10 by menin-MLL1 in HCC.

Figure 4

A. Diagram showing the location of primers at the promoter and downstream regions of PEG10. Genomic coordinates are provided in Supplementary Methods. B. Menin binding to different regions (P-A – P-D) of PEG10 obtained from the chromatin immunoprecipitation (ChiP) experiment performed in HepG2 cells. C, D. ChiP experiments performed in HepG2 cells upon 6 days of treatment with various concentrations of MI-503 or DMSO to detect menin and MLL1 binding as well as H3K4me3 on PEG10. Real-time PCR was performed on the precipitated DNAs with P-B (C) and P-C (D) primers for PEG10 genomic regions. IgG was used as a control. Data represent mean of triplicates ± SD.