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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2017 Nov 20;17(1):39–49. doi: 10.1158/1535-7163.MCT-17-0574

Figure 4. PTC-028 mediated apoptosis is dependent on cellular ATP depletion and mitochondrial ROS.

Figure 4

(A) CP20 or OV90 cells were treated with PTC-028 at 100 nM or with PTC-209 at 200 nm for the indicated times and intracellular ATP levels determined and normalized with respective number of viable cells in each group. Data represent mean ± S.D. of three independent experiments performed in triplicate. *P<0.05 when comparing between each group at respective time point by a two-way ANOVA. (B) OV90 or CP20 cells were treated with 200 nm PTC-209 or 100 nM PTC-028 for 48 h followed by MitoSOX staining and analyzed by a FACS Calibur flow cytometer. A representative histogram depicting mean florescence intensity from three independent replicates is shown. (C) OV90 or CP20 cells were pre-treated with or without Mitoquinone (MQ) 10 μM for 3h followed by PTC-028 at 100 nM for 48 h. Cell viability was assessed by the MTS assay. Vehicle treated control cells were set to 100%. Data represent mean ± S.D. of three independent experiments performed in triplicate and *P<0.05 by a two-way ANOVA. (D) OV90 or CP20 cells were pre-treated with or without Mitoquinone at 10 μM for 3h followed by PTC-028 at 100 nM for 48 h. Expression of XIAP, PARP, cleaved caspase 9 and beta actin was determined by immunoblotting.