Figure 4.

TRF improved motor performance in the Q175 HD model. A, The accelerating rotarod test revealed that the TRF treatment improved motor performance by showing longer latency to fall. B, The challenging beam motor test indicated that the TRF treatment improved performance (fewer errors) by making fewer errors when the mice crossed the balanced beam. C, The circadian parameters and the performance in the two motor tests of individual mouse in ad lib group (black circles) and TRF group (orange triangles) are plotted in a 3D-XYZ grid. In this XYZ grid, there are two distinctive clusters, suggesting that the mouse with stronger circadian rhythms performed better in both motor tests. Comparisons between Q175 cohorts were made using a t test. Asterisks represent significant differences due to TRF regimen compared to ad lib controls (p < 0.05). The correlations between circadian parameters and motor performance are described in the text; n = 8/group.