Figure 4.

Inhibition of SLC29A1 expression promotes hepatocellular carcinoma (HCC) cell growth in vitro and in vivo. Functional analysis, in vitro: A. SLC29A1 expression in MHCC97-H and Huh-7 cells transfected with an shRNA. B. SLC29A1 knockdown in MHCC97-H and Huh-7 cells promoted cell proliferation on day 3. C. The clonogenic ability of HCC cells was enhanced after SLC29A1 inhibition. Functional analysis, in vivo: D. Differences in the expression of epithelial and mesenchymal markers were compared between cancer cells with high and low SLC29A1 expression (MHCC97-H-Mock vs. MHCC97-H-SLC29A1-sh2 and Huh-7-Mock vs. Huh-7-SLC29A1-sh2 cells) using western blot assay. E. The morphological characteristics of tumors in the SLC29A1 shRNA and control groups. F. The differences in tumor weights and volumes between the SLC29A1 shRNA group and control group were statistically significant.