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. 2017 Dec 1;7(12):2526–2535.

Figure 2.

Figure 2

LncRNA HOXD-AS1 knockdown inhibited cell proliferation ability. (A). The lncRNA HOXD-AS1 mRNA expression in B16 cells transfected with pcDNA3.1-vector (vector) and pcDNA3.1-HOXD-AS1 (HOXD-AS1) or in A375 and A2508 cells transfected with si-control, si-HOXD-AS1-1 (siRNA-1) and HOXD-AS1-2 (siRNA-2) were measured by qRT-PCR. (B, C). CCK-8 assays were performed to determine cell growth in B16 cells transfected with pcDNA3.1-vector (vector) and pcDNA3.1-HOXD-AS1 (HOXD-AS1) or in A375 and A2508 cells transfected with si-control, si-HOXD-AS1-1 (siRNA-1) or HOXD-AS1-2 (siRNA-2). (D, E). Colony-forming assays were performed to determine the cloning ability in A375 and A2508 cells transfected with si-control, si-HOXD-AS1-1 (siRNA-1) or HOXD-AS1-2 (siRNA-2) or in B16 cells transfected with pcDNA3.1-vector (vector) and pcDNA3.1-HOXD-AS1 (HOXD-AS1). Data represented the mean ± SD. *, P<0.05.