Knockdown of RUNX3 partly mediated the oncogenic function induced by lncRNA HOXD-AS1. (A) Relative mRNA expression of RUNX3 in A375 cells transfected with si-control and si-RNA-RUNX3. (B-D) CCK8 assays and colony-forming assays were performed to detected the cell viability and cloning ability in A375 cells transfected with si-control, si-RNA-RUNX3, siRNA- HOXD-AS1, and siRNA-HOXD-AS1+siRNA-RUNX3. (E, F) Transwell assays were performed to detected the cell invasion ability in A375 cells transfected with si-control, si-RNA-RUNX3, siRNA-HOXD-AS1, and siRNA-HOXD-AS1+siRNA-RUNX3. Data represented the mean ± SD. *, P<0.05.