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. 2018 Jan 3;4(6):ENEURO.0269-17.2017. doi: 10.1523/ENEURO.0269-17.2017

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Copyright © 2018 Toossi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — c-Fos in RFMes GABAergic neurons across groups. Fluorescent microscopic images show staining for Nissl with FNS (green; A1, B1, C1), immunostaining for GAD (blue; A2, B2, C2, with positive staining indicated by filled arrowheads), and immunostaining for c-Fos (red; A3, B3, C3, with positive staining indicated by filled arrowhead) along with dual staining for Nissl and c-Fos in merged images (green and red; A4, B4, C4, with positive c-Fos staining indicated by filled arrowhead). Note that c-Fos immunostaining is prominent in the nucleus of a GABAergic neuron from an SD mouse (B3, B4), whereas it is not apparent in images from SC or SR mice (A3, A4 or C3, C4, indicated by open arrowheads). Scale bars: 20 μm. Image thickness: 500 nm in all panels.