Figure 5.

Characterization of lysine 2-hydroxyisobutyrylation proteome in HeLa cells. (A) Consensus sequence logo shows a representative sequence for all K_hib sites. (B) Venn diagram shows cellular compartment distribution of 2-hydroxyisobutyrylated proteins. (C) Distribution of the number of K_hib sites on diverse enzymes. The enzymes with < 10 K_hib sites are not shown in this bar graph. (D) Three-dimensional structure of alpha-enolase (ENO1, PDB entry 2PSN) shown with K_hib sites. The important residues in the substrate binding pocked are shown in detail. (E) Table of the K_hib sites on key residues involving substrate or cofactor binding.