FIG 1.

Temperature, pH profile, and kinetic stability of DmmA. (A) A steady increase in the specific activities of DmmA at temperatures ranging from 10 to 35°C with a maximum at 40°C is followed by a rapid loss of activity. Displayed activities are based on an average minimum of three independent reactions with error bars indicating standard deviations of the measurements. (B) Specific activities of DmmA determined at a pH range of 5.3 to 11.6. The displayed profile is based on two independent experiments where the error bars indicate standard deviations. (C) Kinetic stability of DmmA at 45°C at pH 8.0. Each point is an average of the results of three independent experiments. The solid line and error bars represent an exponential fit of the data and the standard deviation, respectively.