Effect of cosolvents on the structure and activity of DmmA. (A) The melting temperature of DmmA was determined in the presence of various concentrations of acetone (diamonds), DMSO (circles), and methanol (squares). (B) The activity of DmmA toward 4-bromobutyronitrile was determined in the presence of various concentrations of acetone (diamonds), DMSO (circles), and methanol (squares). Each point represents an average result of three independent measurements, and the error bar indicates the standard deviation.