TABLE 4.

Studies on improvement of lipase thermostability

Lipase	Design category	Method(s)	Improvement (°C)	No. in screening library	Reference
Bacterial
Bacillus subtilis lipase A	Irrational	DNA shuffling	22.0	18,000	3
Bacillus subtilis lipase LipJ	Irrational	Error-prone PCR	13.0	4,200	16
Pseudomonas aeruginosa lipase	Irrational	Error-prone PCR	2.5	2,600	19
Bacillus subtilis lipase A	Semirational	B-factor analysis	45.0	8,000	4
Geobacillus zalihae T1 lipase	Rational	I-mutant	2.0	2	52
Fungal
Candida antarctica lipase B	Irrational	DNA shuffling	6.4	2,500	17
Rhizopus arrhizus lipase	Irrational	Error-prone PCR, DNA shuffling	10.0	5,500	18
Yarrowia lipolytica lipase Lip2	Semirational	B-factor analysis	3.0	4,200	21
Candida antarctica lipase B	Semirational	B-factor analysis	12.0	2,200	22
Candida rugosa lipase 1	Semirational	B-factor analysis	12.7	20,000	23
Candida antarctica lipase B	Rational	MODIP, DbD v1.20	8.5	5	38
Candida antarctica lipase B	Rational	B-factor analysis, RosettaDesign	2.3	7	31
Rhizomucor miehei lipase	Rational	DbD	3.0	1	37
Rhizomucor miehei lipase	Rational	This study	14.3	38	This study