Effect of glycogen deprivation on sucrose accumulation in strains of Syn7942. Glycogen accumulation (a) and sucrose production (b) by DY126 and FL92 after cultivation in medium containing the indicated concentrations of theophylline are displayed. NaCl (150 mM) and IPTG (1 mM) were added to the medium at day 6, as indicated by the open arrow. T0, T50, T100, and T200 represent 0, 50, 100, and 200 μM theophylline, respectively, supplemented in the medium. Asterisks indicate significant differences from FL92 (Student's t test, P < 0.05).