TABLE 1.
Strain | Genotypea | Cultivation conditions | Titer (mg/liter) | Production rate (mg/liter/h) | Reference |
---|---|---|---|---|---|
Syn7942 strains | |||||
FL92 | NS3::Plac-cscB-Cmr | 30°C, 3% CO2, 100–120 μE/m2/s light, 150 mM NaCl for 3 days | 469.3 | 6.5 | This study |
FL130 | NS1::Ptrc-sps-Spr NS3::Plac-cscB-Cmr | 30°C, 3% CO2, 100–120 μE/m2/s light, 150 mM NaCl for 3 days | 539.8 | 7.5 | This study |
QL241 | NS1::Ptrc-sps-Spr NS2::PcpcB1-glgC-Gmr NS3::Plac-cscB-Cmr | 30°C, 3% CO2, 100–120 μE/m2/s light, 150 mM NaCl for 3 days | 577.8 | 8.0 | This study |
CscB | NS3::Plac-cscB-Cmr | 35°C, 2% CO2, 65 μE/m2/s light, 150 mM NaCl for 3 days | ∼2,016 | ∼28 | 23 |
CscBΔinvA | NS3::Plac-cscB-Cmr invA::Hygr | 35°C, 2% CO2, 65 μE/m2/s light, 150 mM NaCl for 3 days | ∼2,304 | ∼32 | 23 |
CscBΔglgC | NS3::Plac-cscB-Cmr glgC::Spr | 35°C, 2% CO2, 65 μE/m2/s light, 150 mM NaCl for 3 days | ∼2,160 | ∼30 | 23 |
CscBΔglgCΔinvA | NS3::Plac-cscB-Cmr invA::Hygr glgC::Spr | 35°C, 2% CO2, 65 μE/m2/s light, 150 mM NaCl for 3 days | 2,599.2 | 36.1 | 23 |
Syn2973 strain | |||||
Syn2973::CscB | M744_RS12430::Plac-cscB-Cmr | 38°C, 3% CO2, 250 μE/m2/s light, 150 mM NaCl for 4 days | 3,340 | 35.5 | 25 |
Plac, lac promoter; Ptrc, trc promoter; PcpcB1, cpcB1 promoter; Hygr, hygromycin resistance; invA, invertase gene of Syn7942; NS1, NS2, and NS3, different neutral sites in the genome of Syn7942.