TABLE 2.
Strains and oligonucleotides used in this study
| Strain or oligonucleotide | Characteristics or sequence (5′→3′) | Reference |
|---|---|---|
| Syn7942 strains | ||
| FL92 | Starting strain in this study, harboring NS3::cscB, sucrose secreting, Cmr | 24 |
| FL130 | Harboring NS1::Ptrc-sps on the background of FL92, for constructing strain QL241, Cmr Spr | 24 |
| DY126 | Harboring riboswitch-regulated glgC on the background of FL92, Cmr Kmr | This study |
| QL233 | Harboring NS2::PcpcB1-glgC on the background of FL92, Cmr Gmr | This study |
| QL241 | Harboring NS2::PcpcB1-glgC and NS1::Ptrc-sps on the background of FL92, Cmr Gmr Spr | This study |
| Oligonucleotides | ||
| E-F | AAATATTCTGAAATGAGCTGTTGAC | |
| E-glgC-R | ATGATCGCCAGCACGTTTTTCACCTTGTTGCCTCCTTAGCAGGGT | |
| glgC-F | ACCCTGCTAAGGAGGCAACAAGGTGAAAAACGTGCTGGCGATCAT | |
| glgC-R | TTAGATCACCGTGTTGTCGGG | |
| rpe-F | ACTAGTCAGCGTTCACCTCAAGCAACT | |
| rpe-km-R | AGACGTGTAATGCTGCAATCTAATCAATCTCCCCCAAGTCAAG | |
| rpe-km-F | CTTGACTTGGGGGAGATTGATTAGATTGCAGCATTACACGTCT | |
| km-R | ACTAGTGTGACACAGGAACACTTAACGGC | |
| 7942PcpcB1-f | AACACGTGCTACAGCCTGGGTTCCTCATG | |
| 7942PcpcB1-r | CGAGAATGATCGCCAGCACGTTTTTCACTCAACCAGTCTCCTGTTCTCGAC | |
| 7942glgC-f | GTCGAGAACAGGAGACTGGTTGAGTGAAAAACGTGCTGGCGATCATTCTCG | |
| 7942glgC-r | TTAGATCACCGTGTTGTCGG |