Figure 2.

AMD3100 can protect the extracellular function of blood-brain barrier via blocking SDF-1/CXCR4 axis. A, B. The TEER was continuous monitored by Millicell ERS-2 system, and peak value is 180 Ω·cm² after the confluent bEnd.3 cells cultured for 96 hours. After co-cultured with PC-9 cells for 24 h, the value was decreased to 78.7 Ω·cm². C, D. Western blot assays of Zo-1, Occludin and Claudin-5 expression levels in PC-9 cells after treatment with AMD3100, SDF-1, and AMD3100+SDF-1 compared with the blank control. GAPDH was used as the loading control. The bars (fold change) represented the relative expression of proteins to control group. E, F. The numbers of migrated PC-9 cells were counted under fluorescence microscopy after treated with AMD3100 and/or SDF-1 for 24 h.