Figure 8.

In vivo experiments showed that AMD3100 intervention can inhibit CXCR4, VEGF, and MMPS proteins expression in craninocerebral tissues. A. Expression of CXCR4, VEGF, MMP2, and MMP9 were detected by qRT-PCR. Relative mRNA levels are normalized to control. Data are represented as mean ± SEM; n = 3. B. Western blot analysis was used to examine the expression of CXCR4, VEGF, VEGFR2, MMP2, and MMP9 in brain tissues treated with AMD3100 for 24 h. GAPDH was used as a loading control. C, D. Compare of relative mRNA and protein levels of CXCR4, VEGF, MMP2, and MMP9 between 2^nd, 4^th, and 5^th weeks. E, F. The representative images and histograms of relative active MMP2 and MMP9 protein levels in brain tissues were detected by gelatin zymography method at 2^nd, 4^th, and 5^th weeks.