Protein expressions of oxidative stress, apoptotic, mitochondrial, DNA-damaged and energy biogenesis biomarkers in testis by day 28 after Dox treatment. A. Protein expression of NOX-1, * vs. †, p<0.005. B. Protein expression of NOX-2, * vs. †, p<0.001. C. Protein expression of NOX-4, * vs. †, p<0.005. D. Oxidized protein expression, * vs. †, p = 0.01. (Note: left and right lanes shown on the upper panel represent protein molecular weight marker and control oxidized molecular protein standard, respectively). M.W = molecular weight; DNP = 1-3 dinitrophenylhydrazone. n = 6 for each group. Dox = doxorubicin (15 mg/kg). E. Protein expression of mitochondrial (mit)-Bax, * vs. †, p<0.02. F. Protein expression of cleaved caspase (c-Casp) 3, * vs. †, p = 0.001. G. Protein expression of cleaved poly (ADP-ribose) polymerase (PARP), * vs. †, p<0.001. H. Protein expression of γ-H2AX, * vs. †, p = 0.001. I. Protein expression of cytosolic cytochrome (cyt-Cyto) C, * vs. †, p<0.04. J. Protein expression of mitochondrial cytochrome (mit-Cyto) C, * vs. †, p<0.001. K. Protein expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), p = 0.002. n = 6 for each group. Dox = doxorubicin (15 mg/kg).