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. 2017 Dec 15;9(12):5361–5374.

Figure 2.

Figure 2

Mel inhibited migration and increased apoptosis of OSCC-associated neutrophils. (A and B) TANs were treated with or without 1 mM Mel for 1 h. nPBNs without any treatment was used as control. Cell migration was counted 3 h after Mel treatment (A), and cell apoptosis was measured at 24 h after Mel exposure (B, C) nPBNs with or without 1 h of Mel (1 mM) pretreatment were allowed to migrate toward Ctrl-, SCC-9-, or SCC-25-CM for 3 h. (D) nPBNs with or without 1 h of Mel (1 mM) pretreatment were added Ctrl-, SCC-9-, or SCC-25-CM and cultured for 24 h. Cell apoptosis was measured by flow cytometry. All data represent the mean ± SD of three replicates. *P < 0.05 compared with the nPBN group in (A and B) or compared with the nPBN-M or Ctrl-CM group in (C and D). #P < 0.05 compared with the TAN group in (A and B) or compared with the SCC-9- or SCC-25-CM group in (C and D). Ctrl: control; CM: conditioned medium; M: medium; Mel: melatonin.