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. 2017 Dec 15;9(12):5400–5410.

Figure 1.

Figure 1

miR-346 expression inversely correlates with KLF4 expression during NSC differentiation. qPCR analysis of miR-346 (A) and KLF4 mRNA (B) expression in NSCs during differentiation over a period of 7 days. Day 0 represents undifferentiated cells and was normalized to a value of 1. *p < 0.05. (C) Diagram of the predicted miR-346 targeting site within the 3’UTR of KLF4. (D) Bioinformatics analysis of the conservation of the target sequences of miR-346 in the 3’UTR of KLF4 acrossing species. (E) The recombinant pmirGLO vectors containing WT or MT 3’UTR of KLF4 were co-transfected with miR-NC or the miR-346 mimics. *p < 0.05 vs. miR-NC. (F) Correlation of miR-346 expression to KLF4 mRNA expression in NSCs during differentiation. R-squared = 0.9687, p < 0.001. Data were shown as means ± standard deviation (SD) and were processed by one-way ANOVA analysis. Correlations were assessed by Spearman’s rank correlation coefficients. All experiments were repeated at least three times independently.