Figure 4.

miR-346 regulates target genes of KLF4. NSCs were transfected with a scramble miRNA, miR-346 mimics or left untreated (mock). hTERT, LGALS3 and p21 mRNA and protein levels were determined by qPCR (A-C) or western bolt (D-F) respectively 48 h after transfections. For qPCR analysis, GAPDH was served as a internal control. For western bolt analysis, actin was taken as a loading control. Mock cells and was normalized to a value of 1. Data were shown as means ± standard deviation (SD) and were processed by one-way ANOVA analysis. All experiments were repeated at least three times independently. *p < 0.05.