Figure 3.

SUMO1P3 knockdown reduced the migratory and invasive abilities of colon cancer cells in vitro. SW620 and LoVo cells were untransfected (control) and transfected with shSUMO1P3-1 or shNC plasmids. (A) Wound healing assay of SW620 and LoVo cells. Scale bar: 10 μm. (B) Quantification of the wound width in (A) at 0 and 24 h after transfection. (C and E) Transwell assays of the migration (C) and invasion (E) of SW620 and LoVo cells. (D and F) The numbers of migrated (D) and invaded (F) cells were calculated in (C and E) at 24 h after transfection. Data are represented as the mean ± SD of three replicates. *P < 0.05, **P < 0.01 compared with the Ctrl or shNC group. Ctrl: control; shNC: negative control.