Figure 4.

SUMO1P3 silencing inhibited the pro-angiogenesis ability of colon cancer cells in vitro. SW620 and LoVo cells were untransfected (control) and transfected with shSUMO1P3-1 or shNC plasmids. (A) Tube formation assay was performed to evaluate the effect of conditioned media from SW620 and LoVo cells with different treatments on the angiogenesis of HUVECs. (B-D) Relative tube length (B) and number of tubules and branch points (C and D) were counted and calculated. (E) VEGFA levels were determined by using ELISA assays. Data are represented as the mean ± SD of three replicates. *P < 0.05 compared with the Ctrl or shNC group. Ctrl: control; shNC: negative control.