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Metformin attenuates neovascularization in the development of DR through suppressing VEGF-A. (A, B) Vessel density was analyzed at 12 weeks after alloxan, shown by representative images (A), and by quantification (B). (C) ELISA for VEGF-A protein in mouse eyes. (D) RT-qPCR for VEGF-A RNA in mouse eyes. *P<0.05. NS: non-significant. N=10.
