Skip to main content
. 2017 Dec 15;9(12):5558–5566.

Figure 4.

Figure 4

MiR-497a-5p targets 3’-UTR of VEGF-A mRNA to inhibit its expression. A: Bioinformatics analyses show that miR-497a-5p targets VEGF-A mRNA. B: Metformin significantly increased the levels of miR-497a-5p in mouse eyes, shown by RT-qPCR. C: We either overexpressed miR-497a-5p, or inhibited miR-497a-5p in MS1 cells by transfection of the cells with a miR-497a-5p-expressing plasmid, or with a plasmid carrying miR-497a-5p antisense (as-miR-497a-5p). The MS1 cells were also transfected with a null plasmid as a control (null). The overexpression or inhibition of miR-497a-5p in NPC cells was confirmed by RT-qPCR. D: MiR-497a-5p-modified MS1 cells were then transfected with 1 μg of VEGF-A-3’-UTR luciferase-reporter plasmid. The luciferase activities were quantified in these cells. *P<0.05. N=10.