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. 2017 Dec 15;9(12):5619–5630.

Figure 3.

Figure 3

TRPC3/6/7 deletion attenuates albumin excretion and the changes of WT-1, Bcl2, and Cleaved caspase 3 induced by hyperglycemia. A. 24 h albumin excretion was assessed using SDS electrophoresis and staining of gels with Coomassie blue. Both STZ groups developed albuminuria (**p<0.01 and *p<0.05), but albuminuria of TRPC3/6/7-/--STZ mice was significantly less than that of WT-STZ mice (*p<0.05). BSA: bovine serum albumin. n=5-11 mice/group. B. Western blot showed that WT-1 protein of the renal cortex was decreased obviously in WT-STZ mice, compared with WT-Veh mice (**p<0.01); however, the decrease were prevented in TRPC3/6/7-/- mice (*p<0.05). n=3-5 mice/group. C. Western blot analysis demonstrated that the downregulation of Bcl2 and upregulation of Cleaved caspase 3 in WT-STZ mice (***p<0.001 and **p<0.01) was prevented in TRPC3/6/7-/--STZ mice (***p<0.001 and *p<0.05). n=3-5 mice/group.