Figure 4.

TRPC3/6/7 deletion inhibits the activation of TGFβ1 signaling and fibronectin deposition in diabetic kidney. A. Western blot analysis showed that TGFβ1 and p-Smad2/3 of renal cortex of WT-STZ mice were increased compared with WT-Veh mice (**p<0.01 and *p<0.05); however, the increase was inhibited in TRPC3/6/7^-/--STZ mice (both *p<0.05). n=3-5 mice/group. B. Immunohistochemistry showed that the deposition of TGFβ1 and Fibronectin in renal cortex of WT-STZ mice were not obvious in TRPC3/6/7^-/--mice. n=3-5 mice/group.