FIG 6.

HCV infection upregulates MYC and reduces NDRG1 phosphorylation. (A) HCV replication upregulates MYC. Huh7.5.1 cells were infected with HCV, and total RNA was harvested at the indicated intervals. MYC expression was measured by RT-qPCR. Uninfected cells were used as a control. (B) HCV replication does not upregulate NMYC. Huh7.5.1 cells were infected with HCV and harvested as described above for panel A. NMYC expression was detected by RT-qPCR. Uninfected cells were used as a control. (C) Quantitative Western blot analysis of HCV-infected cells. Huh7.5.1 cells were infected with HCV, and total protein was harvested at the indicated time points. Protein levels were quantified by using the Wes system, except for NS3, which was quantified by a traditional Western method. Uninfected cells were harvested in parallel as a control. Error bars denote standard deviations. All experiments were performed three times, and the data from a representative experiment are shown. **, P ≤ 0.01; *, P ≤ 0.05 (compared to the negative controls). In panel C, graphs represent total peak intensity as measured by the instrument.