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Titration of CD4-Ig binding to Env trimers. CD4-Ig binding to mac-tropic and non-mac-tropic R5 Envs was titrated out. Mac-tropic B33, JRFL, B93, and CA110 1-2 Env binding to CD4-Ig was detectable at <1 μg/ml, while binding to non-mac-tropic Envs LN40 and LN33 was undetectable at 10 μg/ml. However, weak, low-level binding of CD4-Ig to non-mac-tropic JRCSF was apparent.
