FIG 10.

Proposed model for the miR-3570 regulatory mechanism in fish species. (A) Schematic diagram of the predicted target sites of miR-3570 in the 3′UTR of Danio rerio MAVS. HEK293 cells were transfected with miR-3570 or Ctrl, along with the wild-type Danio rerio MAVS-3′UTR (DrMAVS 3′UTR WT) or the mutant type of Danio rerio MAVS-3′UTR (DrMAVS 3′UTR MT) for 24 h, and the luciferase activity was determined. (B) Schematic diagram of the predicted target sites of miR-3570 in 3′UTR of Larimichthys crocea MAVS. HEK293 cells were transfected with miR-3570 or Ctrl, along with the wild-type Larimichthys crocea MAVS-3′UTR (LcMAVS 3′UTR WT) or the mutant type of Larimichthys crocea MAVS-3′UTR (LcMAVS 3′UTR MT) for 24 h, and the luciferase activity was determined. (C) Proposed model for the mechanism by which induced miR-3570 negatively regulates inflammatory cytokines and antiviral gene production in feedback manner by targeting MAVS and inhibiting NF-κB and IRF3 signaling, thereby promoting virus replication.