FIG 9.

miR-3570 promotes SCRV replication. (A) Miiuy croaker macrophages were transfected with Ctrl, miR-3570, Ctrl-i, or miR-3570-i, infected by SCRV at an MOI of 5 for 1 h, washed, and then added to fresh medium. After 72 h, SCRV TCID₅₀ in cultural supernatants was measured with MKC cells. (B and C) qRT-PCR analysis was conducted for intracellular SCRV RNA (B) or supernatant SCRV RNA (C). All data are representative of at least three independent experiments (**, P < 0.01; *, P < 0.05; N.D., not detected).