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Neutralization assay of HAdV-4 with polyclonal antisera from mice immunized with epitope-chimeric adenoviruses. The antibody-virus mixtures were transferred to 96-well plates containing 85% to 95% confluent monolayers of AD293 cells. After culture for 96 h, the HAdV-4 genome copy numbers were determined by Q-PCR. Mouse anti-rAd3 and anti-Ad4 sera were used as the controls.
