FIG 5.

Confirmation of the epitope recognized by MN4b with mutations in HVR7 of HAdV-4. (A) The sequences shown represent HVR7 of HAdV-4 and HAdV-3 and the mutations in HVR7 of HAdV-4 hexon. Mutated residues are shown in gray. (B) Indirect ELISAs were performed to assess the reactions of serially diluted MN4b with HAdV-4 and HAdV-4 mutants containing chimeric hexon. (C) Immunoblot analyses of HAdV-4 (Ad4) (lanes 1 and 5), chimeric rAd4-3R7-1 (lanes 2 and 6), rAd4-3R7-2 (lanes 3 and 7), and rAd4-3R7 (lanes 4 and 8) were performed with MN4b (lanes 5 to 8) and antiserum from mice immunized with HAdV-4 hexon (lanes 1 to 4). (D) Neutralizing titers of MN4b that inhibited infection of AD293 cells by HAdV-4, rAd4-3R7-1, rAd4-3R-2, or rAd3E were determined by neutralization assays. MAb 3D7, a neutralizing antibody directed against HAdV-3, was used as a control. Antisera that showed no reactivity at a 1:16 dilution (the lowest dilution tested) were assigned a titer of 1:4.