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HAdV-4 R7-1 mutants partially escape neutralization by anti-HAdV-4 sera from mice and rabbits. (A) Neutralization of HAdV-4 mutants in AD293 cells by sera from HAdV-4-immunized mice or rabbits was measured by neutralization assays. Antisera that showed no reactivity at a 1:16 dilution (the lowest dilution tested) were assigned a titer of 1:4. (B and C) The neutralization assay results were confirmed by detection of the adenovirus genome copy numbers.
